{"id":339,"date":"2021-03-19T08:23:11","date_gmt":"2021-03-19T08:23:11","guid":{"rendered":"http:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/?page_id=339"},"modified":"2021-03-19T08:23:11","modified_gmt":"2021-03-19T08:23:11","slug":"molecular-basis-of-human-channelopathies-molcan","status":"publish","type":"page","link":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/molecular-basis-of-human-channelopathies-molcan\/","title":{"rendered":"Molecular basis of human channelopathies (MOLCAN)"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text]<\/p>\n

\n

Molecular basis of human channelopathies (MOLCAN)<\/h1>\n<\/div>\n
\n

PRINCIPAL INVESTIGATOR<\/h4>\n\n\n\n
<\/td>\nTeresa Gir\u00e1ldez Fern\u00e1ndez, PhD<\/strong><\/p>\n

Lecturer in Physiology, Department of Basic Medical Sciences, ULL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

POSTDOCTORAL RESEARCHERS:<\/h4>\n
    \n
  • Alejandro Cerrada de Due\u00f1as, PhD (Postdoctoral researcher, contracted by ERC project until September 2019, ULL)<\/li>\n
  • Aravind Kshatri, PhD (\u201cJuan de la Cierva\u201d researcher, Department of Basic Medical Sciences, ULL)<\/li>\n
  • David Bartolom\u00e9 Mart\u00edn, PhD (Postdoctoral researcher, contracted by ERC project, ULL)<\/li>\n
  • Ricardo G\u00f3mez Garc\u00eda, PhD (Postdoctoral researcher, contracted by ERC project, ULL)<\/li>\n
  • Laura E. Maglio, PhD (Postdoctoral researcher, contracted by ERC Project, ULL)<\/li>\n
  • Belinda Rivero, PhD (Postdoctoral researcher, \u00abAgust\u00edn de Betencourt\u00bb programme, Department of Basic Medical Sciences, ULL)<\/li>\n<\/ul>\n

    PHD STUDENTS AND TRAINEES:<\/h4>\n
      \n
    • Alberto J. Gonz\u00e1lez Hern\u00e1ndez (funded by FPU, ULL)<\/li>\n
    • Elva Mart\u00edn Batista (funded by FPU, ULL)<\/li>\n
    • Rebeca Mart\u00ednez L\u00e1zaro (Master’s project and PhD student)<\/li>\n<\/ul>\n

      TECHNICAL AND ADMINISTRATIVE STAFF:<\/h4>\n
        \n
      • Nair Mesejo Nores (Project manager, contracted by ERC Project, ULL)<\/li>\n<\/ul>\n

        RESEARCH LINES:<\/h4>\n

        Teresa Gir\u00e1ldez\u2019s group is interested in the structure-function of ion channels involved in controlling neuronal excitability, and on their role in different neurological and psychiatric diseases (epilepsy, fragile X syndrome, autism, etc.). They employ cellular and molecular biology, and biochemical techniques, as well as advanced microscopy and fluorescence methods (confocal and super-resolution microscopy). These methods are combined with conventional and novel electrophysiological approaches, such as patch-clamp, two electrode voltage clamp and patch-clamp fluorimetry. In the mid-term, the group\u2019s objectives are: (i) to reveal novel regulatory mechanisms of calcium nanodomains using different sources of Ca2+<\/sup>\u00a0(presynaptic voltage-gated calcium channels and postsynaptic NMDAR receptors); (ii) to understand the role of NMDAR-BK complexes in neuronal plasticity and the development of seizures; (iii) to establish new animal models bearing endogenous calcium sensor nanodomains based on \u201cbig\u201d potassium channels (BK) previously developed in our laboratory.<\/p>\n

        FUNDED PROJECTS:<\/h4>\n
          \n
        • Optoelectrical dynamics of ion channel activation and calcium nanodomains (NANOPDICS; ERC-CoG-2014). PI: Teresa Gir\u00e1ldez. European Research Council (ERC Consolidator Grant; 2015-2020, 1,999,742 \u20ac).<\/li>\n
        • Pathophysiological roles of NMDAR-BK complexes in the brain (NeuroGluNBK, RTI2018-098768-B-C21). IPI: Teresa Gir\u00e1ldez. National I+D+i (R+D+I) programme directed at challenges in society. MINECO (2019-2021, 190,000 \u20ac + 1 PhD studentship FPI)<\/li>\n
        • Molecular mechanisms underlying the neuroprotective and anti-seizure activity of the neuronal SGK1.1 kinase through Kv7 ion channels (SGK-EPIK; BFU2015-66490-R \u2013 \u201cMecanismos moleculares subyacentes al papel neuroprotector y anticonvulsionante de la quinasa neuronal SGK1.1 mediante regulaci\u00f3n de canales i\u00f3nicos Kv7\u201d). PI: Teresa Gir\u00e1ldez. MINECO (National I+D+i (R+D+I) programme directed at challenges in society; 2016-2019 157,300 \u20ac+ 1 PhD studentship FPI).<\/li>\n
        • National \u201cRam\u00f3n y Cajal\u201d Programme. Postdoctoral contracts for Research and Development centres (RYC-2012-11349). PI: Teresa Gir\u00e1ldez. MINECO (Ram\u00f3n y Cajal Sub-programme; 2014-2019, 268,600 \u20ac 5 year salary + 40,000 \u20ac Research Project).<\/li>\n<\/ul>\n

          PUBLICATIONS 2019:<\/h4>\n
            \n
          • Armas-Capote N, Maglio LE, P\u00e9rez-Atencio L, Martin-Batista E, Reboreda A, Barios JA, Hernandez G, Alvarez de la Rosa D, Lamas JA, Barrio LC, Giraldez T. SGK1.1 Reduces Kainic Acid-Induced Seizure Severity and Leads to Rapid Termination of Seizures. Cereb Cortex. 2019 Dec 9. pii: bhz302. doi: 10.1093\/cercor\/bhz302<\/li>\n
          • Noriega-Prieto JA,Maglio LE,Gallero-Salas Y,Fern\u00e1ndez de Sevilla D. Nitric Oxide-Dependent LTD at Infralimbic Cortex. 2019 Oct 15;418:149-156. doi: 10.1016\/j.neuroscience.2019.08.029<\/li>\n
          • Bartolom\u00e9-Mart\u00edn D, Ib\u00e1\u00f1ez I, Piniella D, Mart\u00ednez-Blanco E, Pelaz SG, Zafra F. Identification of potassium channel proteins Kv7.2\/7.3 as common partners of the dopamine and glutamate transporters DAT and GLT-1. Neuropharmacology. 2019 Dec 15;161:107568. doi: 10.1016\/j.neuropharm.2019.03.011<\/li>\n
          • Ib\u00e1\u00f1ez I, Bartolom\u00e9-Mart\u00edn D, Piniella D, Gim\u00e9nez C, Zafra F. Activity dependent internalization of the glutamate transporter GLT-1 requires calcium entry through the NCX sodium\/calcium exchanger. Neurochem Int. 2019 Feb;123:125-132. doi: 10.1016\/j.neuint.2018.03.012<\/li>\n
          • Ramp\u00e9rez A, Bartolom\u00e9-Mart\u00edn D, Garc\u00eda-Pascual A, S\u00e1nchez-Prieto J, Torres M. Photoconversion of FM1-43 Reveals Differences in Synaptic Vesicle Recycling and Sensitivity to Pharmacological Disruption of Actin Dynamics in Individual Synapses. ACS Chem Neurosci. 2019 Apr 17;10(4):2045-2059. doi: 10.1021\/acschemneuro.8b00712<\/li>\n<\/ul>\n

            OTHER SELECTED PUBLICATIONS FROM THE LAST 10 YEARS:<\/h4>\n
              \n
            • Miranda P, Holmgren M, Gir\u00e1ldez T. Voltage-dependent dynamics of the BK channel cytosolic gating ring are coupled to the membrane-embedded voltage sensor. Elife. 2018. 11;7. pi: e40664. doi: 10.7554\/eLife.40664<\/li>\n
            • Miranda P, Gir\u00e1ldez T, Holmgren M. Interactions of divalent cations with calcium binding sites of BK channels reveal independent motions within the gating ring. Proc Natl Acad Sci U. S. A. 2016 Dec 6;113(49):14055-14060. doi: 1073\/pnas.1611415113<\/li>\n
            • Gonzalez-Montelongo R, Barros F, \u00c1lvarez de la Rosa D, Gir\u00e1ldez T. Plasma membrane insertion of epithelial sodium channels occurs with dual kinetics. Pflugers Arch. 2016 May;468(5):859-70. doi: 10.1007\/s00424-016-1799-4.<\/li>\n
            • Miranda P, Contreras JE, Plested AJ, Sigworth FJ, Holmgren M, Gir\u00e1ldez T. State-dependent FRET reports calcium-and voltage-dependent gatingring motions in BK channels. Proc Natl Acad Sci U.S.A. 2013 Mar 26;110(13):5217-22. doi: 10.1073\/pnas.1219611110<\/li>\n
            • Miranda P, Cadaveira-Mosquera A, Gonzalez-Montelongo R, Villaroel A, Gonz\u00e1lez-Hern\u00e1ndez T, Lamas JA, \u00c1lvarez de la Rosa D, Gir\u00e1ldez T. The neuronal serum and glucocorticoid regulated kinase 1.1 reduces neuronal excitability and protects against seizures through upregulation of the M-current. J Neurosci. 2013 Feb 6;33(6):2684-96. doi: 10.1523\/JNEUROSCI.3442-12.2013<\/li>\n<\/ul>\n<\/div>\n

              [\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"

              [vc_row][vc_column][vc_column_text] Molecular basis of human channelopathies (MOLCAN) PRINCIPAL INVESTIGATOR Teresa Gir\u00e1ldez Fern\u00e1ndez, PhD Lecturer in Physiology, Department of Basic Medical Sciences, ULL POSTDOCTORAL RESEARCHERS: Alejandro Cerrada de Due\u00f1as, PhD (Postdoctoral researcher, contracted by ERC project until September 2019, ULL) Aravind Kshatri, PhD (\u201cJuan de la Cierva\u201d researcher, Department of Basic Medical Sciences, ULL) David Bartolom\u00e9…<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"templates\/template-no-title.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/pages\/339"}],"collection":[{"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/comments?post=339"}],"version-history":[{"count":1,"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/pages\/339\/revisions"}],"predecessor-version":[{"id":340,"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/pages\/339\/revisions\/340"}],"wp:attachment":[{"href":"https:\/\/www.ull.es\/institutos\/institute-biomedical-technologies\/wp-json\/wp\/v2\/media?parent=339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}