Cloning
For gene editing experiments with insertions of specific sequences, a DNA repair template is needed which, depending on its size, must be created by cloning, which can be requested from the service.

Obtaining a genetically modified cell line
This service includes all the necessary steps to establish a genetically modified cell line, from the transfection of the reagents to obtaining the clone with the desired modification, including obtaining different clones and screening them.

Pronuclear microinjection (soon)
Service to inject the content needed by the user into the pronucleus of a fertilized mouse egg by microinjection.

In vitro fertilization (soon)
The in vitro fertilization process is essential for the recovery or creation of a mouse model. The fertilized embryos must then be implanted in a surrogate mother, unless the objective is to study their development to the blastocyst stage.

Embryo transfer (soon)
Embryo transfer to surrogate mothers is necessary both when creating a new mouse model and when acquiring a commercially available mouse model that is transported cryopreserved. In the latter case, it not only facilitates air transport but also prevents the introduction of pathogens into the animal facility.

Cryopreservation of mouse sperm (soon)
Mouse sperm preservation is ensured by freezing it in liquid nitrogen.

Cryopreservation of mouse embryos (soon)
Preservation of mouse embryos by freezing them in liquid nitrogen.

Liquid nitrogen maintenance (soon)
Once the sperm and embryos are cryopreserved, they must be kept at an adequate level of nitrogen for proper maintenance.

Thawing mouse embryos (soon)
This service offers the possibility of thawing mouse embryos for revitalization.

Mouse genotyping
When working with mouse models, it is necessary to genotype them to confirm that they retain the genetic modification they initially carried. Genotyping is also necessary after a gene-editing experiment.