Drug Delivery Systems


Garcia-Garcia P, Reyes R, Rodriguez JA, Martín T, Evora C, Diaz-Rodriguez P, Delgado A. The Bone Regeneration Capacity of BMP-2 + MMP-10 Loaded Scaffolds Depends on the Tissue Status. Pharmaceutics. 2021; 13(7): 979. https://doi.org/10.3390/pharmaceutics13070979

Diaz-Rodriguez P, Mariño C, Vázquez JA, Caeiro-Rey JR, Landin M. Targeting joint inflammation for osteoarthritis management through stimulus-sensitive hyaluronic acid based intra-articular hydrogels. Materials Science and Engineering: C . 2021; 112254. https://doi.org/10.1016/j.msec.2021.112254

Garcia-Garcia P, Briffault E, Landin M, Evora C, Diaz-Rodriguez P, Delgado A. Tailor-made oligonucleotide-loaded lipid-polymer nanosystems designed for bone gene therapy. Drug Delivery and Translational Research. 2021; 11: 598–607. https://doi.org/10.1007/s13346-021-00926-5

Hreha TN, Foreman S, Duran-Pinedo A, Morris AR, Diaz-Rodriguez P, Jones JA, Ferrara K, Bourges A, Rodriguez L, Koffas MAG, Hahn M, Hauser AR, Barquera B. The three NADH dehydrogenases of Pseudomonas aeruginosa: Their roles in energy metabolism and links to virulence. PLoS ONE. 2021; 16(2): e0244142. https://doi.org/10.1371/journal.pone.0244142

Garcia-del Rio L, Diaz-Rodriguez P, Landin M. Design of novel orotransmucosal vaccine-delivery platforms using artificial intelligence. European Journal of Pharmaceutics and Biopharmaceutics. 2021; 159: 36-43. https://doi.org/10.1016/j.ejpb.2020.12.018

Rouco H, Diaz-Rodriguez P, Gaspar DP, Gonçalves LMD, Cuerva M, Remuñán-López C, Almeida AJ, Landin M. Rifabutin-Loaded Nanostructured Lipid Carriers as a Tool in Oral Anti-Mycobacterial Treatment of Crohn’s Disease. Nanomaterials. 2020; 10(11): 2138. https://doi.org/10.3390/nano10112138

García-García P, Reyes R, Pérez-Herrero E, Arnau MR, Évora C, Delgado A. Alginate-hydrogel versus alginate-solid system. Efficacy in bone regeneration in osteoporosis. Mater Sci Eng C Mater Biol Appl. 2020; 115: 111009. https://doi.org/10.1016/j.msec.2020.111009

Lorenzo-Veiga B, Diaz-Rodriguez P, Alvarez-Lorenzo C, Loftsson T, Sigurdsson HH. In vitro and ex vivo evaluation of nepafenac-based cyclodextrin microparticles for treatment of eye inflammation. Nanomaterials. 2020; 10: 709. https://doi.org/10.3390/nano10040709

Salas A, López J, Reyes R, Évora C, Montes de Oca F, Báez D, Delagado A, Almeida TA. Organotypic culture as a research and preclinical model to study uterine leiomyomas. Scientific Reports. 2020; 10: 5212. https://doi.org/10.1038/s41598-020-62158-w

García-García P, Reyes R, Segredo-Morales E, Pérez-Herrero E, Delgado A, Évora C. PLGA-BMP-2 and PLA-17β-Estradiol Microspheres Reinforcing a Composite Hydrogel for Bone Regeneration in Osteoporosis. Pharmaceutics. 2019;11(12). pii:E648. https://doi.org/10.3390/pharmaceutics11120648

García-García P, Ruiz M, Reyes R, Delgado A, Évora C, Riancho JA, Rodríguez-Rey JC, Pérez-Campo FM. Smurf1 Silencing Using a LNA-ASOs/Lipid Nanoparticle System to Promote Bone Regeneration. Stem Cells Transl Med. 2019;8(12):1306-1317. https://doi.org/10.1002/sctm.19-0145

Pérez-Herrero E, García-García P, Gómez-Morales J, Llabrés M, Delgado A, Évora C. New injectable two-step forming hydrogel for delivery of bioactive substances in tissue regeneration. Regen Biomater. 2019;6(3):149-162. https://doi.org/10.1093/rb/rbz018

García-Orue I, Gainza G, García-García P, Gutierrez FB, Aguirre JJ, Hernandez RM, Delgado A, Igartua M. Composite nanofibrous membranes of PLGA/Aloe vera containing lipid nanoparticles for wound dressing applications. Int J Pharm. 2019;556:320-329. https://doi.org/10.1016/j.ijpharm.2018.12.010

Vayas R, Reyes R, Arnau MR, Évora C, Delgado A. Injectable Scaffold for Bone Marrow Stem Cells and Bone Morphogenetic Protein-2 to Repair Cartilage. Cartilage. 2019:1947603519841682. https://doi.org/10.1177/1947603519841682

Segredo-Morales E, Reyes R, Arnau MR, Delgado A, Évora C. In situ gel-forming system for dual BMP-2 and 17β-estradiol controlled release for bone regeneration in osteoporotic rats. Drug Deliv Transl Res. 2018;8(5):1103-1113. https://doi.org/10.1007/s13346-018-0574-9

Segredo-Morales E, García-García P, Reyes R, Pérez-Herrero E, Delgado A, Évora C. Bone regeneration in osteoporosis by delivery BMP-2 and PRGF from tetronic-alginate composite thermogel. Int J Pharm. 2018;543(1-2):160-168. https://doi.org/10.1016/j.ijpharm.2018.03.034

Reyes R, Rodríguez JA, Orbe J, Arnau MR, Évora C, Delgado A. Combined sustained release of BMP2 and MMP10 accelerates bone formation and mineralization of calvaria critical size defect in mice. Drug Deliv. 2018;25(1):750-756. https://doi.org/10.1080/10717544.2018.1446473

Segredo-Morales E, Martin-Pastor M, Salas A, Évora C, Concheiro A, Alvarez-Lorenzo C, Delgado A. Mobility of Water and Polymer Species and Rheological Properties of Supramolecular Polypseudorotaxane Gels Suitable for Bone Regeneration. Bioconjug Chem. 2018;29(2):503-516. https://doi.org/10.1021/acs.bioconjchem.7b00823

Santoveña A, Monzón C, Alvarez-Lorenzo C, Del Rosario C, Delgado A, Evora C, Concheiro A, Llabrés M, Fariña JB. Structure-Performance Relationships of Temperature-Responsive PLGA-PEG-PLGA Gels for Sustained Release of Bone Morphogenetic Protein-2. J Pharm Sci. 2017;106(11):3353-3362. https://doi.org/10.1016/j.xphs.2017.07.007

Vayas R, Reyes R, Rodríguez-Évora M, Del Rosario C, Delgado A, Évora C. Evaluation of the effectiveness of a bMSC and BMP-2 polymeric trilayer system in  cartilage repair. Biomed Mater. 2017;12(4):045001. https://doi.org/10.1088/1748-605X/aa6f1c

Diaz-Gomez L, García-González CA, Wang J, Yang F, Aznar-Cervantes S, Cenis JL, Reyes R, Delgado A, Évora C, Concheiro A, Alvarez-Lorenzo C. Biodegradable PCL/fibroin/hydroxyapatite porous scaffolds prepared by supercritical foaming for bone regeneration. Int J Pharm. 2017;527(1-2):115-125. https://doi.org/10.1016/j.ijpharm.2017.05.038

Abellan-Pose R, Rodríguez-Évora M, Vicente S, Csaba N, Évora C, Alonso MJ, Delgado A. Biodistribution of radiolabeled polyglutamic acid and PEG-polyglutamic acid nanocapsules. Eur J Pharm Biopharm. 2017;112:155-163. https://doi.org/10.1016/j.ejpb.2016.11.015

Garcia-Orue I, Gainza G, Gutierrez FB, Aguirre JJ, Evora C, Pedraz JL, Hernandez RM, Delgado A, Igartua M. Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications. Int J Pharm. 2017;523(2):556-566. https://doi.org/10.1016/j.ijpharm.2016.11.006

Del Rosario C, Rodríguez-Évora M, Reyes R, Simões S, Concheiro A, Évora C, Alvarez-Lorenzo C, Delgado A. Bone critical defect repair with poloxamine-cyclodextrin supramolecular gels. Int J Pharm. 2015;495(1):463-473. https://doi.org/10.1016/j.ijpharm.2015.09.003

Del Rosario C, Rodríguez-Évora M, Reyes R, Delgado A, Évora C. BMP-2, PDGF-BB, and bone marrow mesenchymal cells in a macroporous β-TCP scaffold for critical-size bone defect repair in rats. Biomed Mater. 2015;10(4):045008. https://doi.org/10.1088/1748-6041/10/4/045008

Del Rosario C, Rodríguez-Evora M, Reyes R, González-Orive A, Hernández-Creus A, Shakesheff KM, White LJ, Delgado A, Evora C. Evaluation of nanostructure and microstructure of bone regenerated by BMP-2-porous scaffolds. J Biomed Mater Res A. 2015;103(9):2998-3011. https://doi.org/10.1002/jbm.a.35436

Rodríguez-Evora M, Reyes R, Alvarez-Lorenzo C, Concheiro A, Delgado A, Evora C. Bone regeneration induced by an in situ gel-forming poloxamine, bone morphogenetic protein-2 system. J Biomed Nanotechnol. 2014;10(6):959-69. https://doi.org/10.1166/jbn.2014.1801

Rodríguez-Évora M, García-Pizarro E, del Rosario C, Pérez-López J, Reyes R, Delgado A, Rodríguez-Rey JC, Évora C. Smurf1 knocked-down, mesenchymal stem cells and BMP-2 in an electrospun system for bone regeneration. Biomacromolecules. 2014;15(4):1311-22. https://doi.org/10.1021/bm401854d

Alonso-Sande M, des Rieux A, Fievez V, Sarmento B, Delgado A, Evora C, Remuñán-López C, Préat V, Alonso MJ. Development of PLGA-mannosamine nanoparticles as oral protein carriers. Biomacromolecules. 2013;14(11):4046-52. https://doi.org/10.1021/bm401141u

Reyes R, Pec MK, Sánchez E, del Rosario C, Delgado A, Évora C. Comparative, osteochondral defect repair: stem cells versus chondrocytes versus bone morphogenetic protein-2, solely or in combination. Eur Cell Mater. 2013;25:351-65. https://doi.org/10.22203/ecm.v025a25

Rodríguez-Évora M, Delgado A, Reyes R, Hernández-Daranas A, Soriano I, San Román J, Evora C. Osteogenic effect of local, long versus short term BMP-2 delivery from a novel SPU-PLGA-βTCP concentric system in a critical size defect in rats. Eur J Pharm Sci. 2013;49(5):873-84. https://doi.org/10.1016/j.ejps.2013.06.008 

Reyes R, Delgado A, Solis R, Sanchez E, Hernandez A, San Roman J, Evora C. Cartilage repair by local delivery of transforming growth factor-β1 or bone morphogenetic protein-2 from a novel, segmented polyurethane/polylactic-co-glycolic bilayered scaffold. J Biomed Mater Res A. 2014;102(4):1110-20. https://doi.org/10.1002/jbm.a.34769

Beloqui A, Solinís MÁ, Delgado A, Evora C, Isla A, Rodríguez-Gascón A. Fate of nanostructured lipid carriers (NLCs) following the oral route: design, pharmacokinetics and biodistribution. J Microencapsul. 2014;31(1):1-8. https://doi.org/10.3109/02652048.2013.788090

Beloqui A, Solinís MA, Delgado A, Evora C, del Pozo-Rodríguez A, Rodríguez-Gascón A. Biodistribution of Nanostructured Lipid Carriers (NLCs) after intravenous administration to rats: influence of technological factors. Eur J Pharm Biopharm. 2013;84(2):309-14. https://doi.org/10.1016/j.ejpb.2013.01.029

Reyes R, Delgado A, Sánchez E, Fernández A, Hernández A, Evora C. Repair of an osteochondral defect by sustained delivery of BMP-2 or TGFβ1 from a bilayered  alginate-PLGA scaffold. J Tissue Eng Regen Med. 2014;8(7):521-33. https://doi.org/10.1002/term.1549

Delgado JJ, Sánchez E, Baro M, Reyes R, Evora C, Delgado A. A platelet derived growth factor delivery system for bone regeneration. J Mater Sci Mater Med. 2012;23(8):1903-12. https://doi.org/10.1007/s10856-012-4661-z

Hernández A, Reyes R, Sánchez E, Rodríguez-Évora M, Delgado A, Evora C. In vivo osteogenic response to different ratios of BMP-2 and VEGF released from a biodegradable porous system. J Biomed Mater Res A. 2012;100(9):2382-91. https://doi.org/10.1002/jbm.a.34183

Reyes R, De la Riva B, Delgado A, Hernández A, Sánchez E, Évora C. Effect of triple growth factor controlled delivery by a brushite-PLGA system on a bone defect. Injury. 2012;43(3):334-42. https://doi.org/10.1016/j.injury.2011.10.008

Hernández A, Sánchez E, Soriano I, Reyes R, Delgado A, Évora C. Material-related effects of BMP-2 delivery systems on bone regeneration. Acta Biomater. 2012;8(2):781-91. https://doi.org/10.1016/j.actbio.2011.10.008

Pec MK, Reyes R, Sánchez E, Carballar D, Delgado A, Santamaría J, Arruebo M, Evora C. Reticulated vitreous carbon: a useful material for cell adhesion and tissue invasion. Eur Cell Mater. 2010; 20:282-93. https://doi.org/10.22203/ecm.v020a23

De la Riva B, Sánchez E, Hernández A, Reyes R, Tamimi F, López-Cabarcos E, Delgado A, Evora C. Local controlled release of VEGF and PDGF from a combined brushite-chitosan system enhances bone regeneration. J Control Release. 2010;143(1):45-52. https://doi.org/10.1016/j.jconrel.2009.11.026

De la Riva B, Nowak C, Sánchez E, Hernández A, Schulz-Siegmund M, Pec MK, Delgado A, Evora C. VEGF-controlled release within a bone defect from alginate/chitosan/PLA-H scaffolds. Eur J Pharm Biopharm. 2009;73(1):50-8. https://doi.org/10.1016/j.ejpb.2009.04.014

Alvarez H, Castro C, Moujir L, Perera A, Delgado A, Soriano I, Evora C, Sánchez E. Efficacy of ciprofloxacin implants in treating experimental osteomyelitis. J Biomed Mater Res B Appl Biomater. 2008;85(1):93-104. https://doi.org/10.1002/jbm.b.30921

Delgado JJ, Evora C, Sánchez E, Baro M, Delgado A. Validation of a method for non-invasive in vivo measurement of growth factor release from a local delivery system in bone. J Control Release. 2006;114(2):223-9. https://doi.org/10.1016/j.jconrel.2006.05.026

Soriano I, Martín AY, Evora C, Sánchez E. Biodegradable implantable fluconazole delivery rods designed for the treatment of fungal osteomyelitis: influence of gamma sterilization. J Biomed Mater Res A. 2006;77(3):632-8. https://doi.org/10.1002/jbm.a.30657

Castro C, Evora C, Baro M, Soriano I, Sánchez E. Two-month ciprofloxacin implants for multibacterial bone infections. Eur J Pharm Biopharm. 2005;60(3):401-6. https://doi.org/10.1016/j.ejpb.2005.02.005

Vila A, Sánchez A, Evora C, Soriano I, McCallion O, Alonso MJ. PLA-PEG particles as nasal protein carriers: the influence of the particle size. Int J Pharm. 2005;292(1-2):43-52. https://doi.org/10.1016/j.ijpharm.2004.09.002

Vila A, Sánchez A, Evora C, Soriano I, Vila Jato JL, Alonso MJ. PEG-PLA nanoparticles as carriers for nasal vaccine delivery. J Aerosol Med. 2004;17(2):174-85. https://doi.org/10.1089/0894268041457183

Negrín CM, Delgado A, Llabrés M, Evora C. Methadone implants for methadone maintenance treatment. In vitro and in vivo animal studies. J Control Release. 2004;95(3):413-21. https://doi.org/10.1016/j.jconrel.2003.12.008

Castro C, Sánchez E, Delgado A, Soriano I, Núñez P, Baro M, Perera A, Evora C. Ciprofloxacin implants for bone infection. In vitro-in vivo characterization. J Control Release. 2003;93(3):341-54. https://doi.org/10.1016/j.jconrel.2003.09.004

Baro M, Sánchez E, Delgado A, Perera A, Evora C. In vitro-in vivo characterization of gentamicin bone implants. J Control Release. 2002;83(3):353-64. https://doi.org/10.1016/S0168-3659(02)00179-7

Sánchez E, Baro M, Soriano I, Perera A, Evora C. In vivo-in vitro study of biodegradable and osteointegrable gentamicin bone implants. Eur J Pharm Biopharm. 2001;52(2):151-8. https://doi.org/10.1016/S0939-6411(01)00169-2

Negrin CM, Delgado A, Llabrés M, Evora C. In vivo-in vitro study of biodegradable methadone delivery systems. Biomaterials. 2001;22(6):563-70. https://doi.org/10.1016/S0142-9612(00)00214-3

Delgado A, Soriano I, Sánchez E, Oliva M, Evora C. Radiolabelled biodegradable microspheres for lung imaging. Eur J Pharm Biopharm. 2000;50(2):227-36. https://doi.org/10.1016/S0939-6411(00)00109-0

Tobío M, Sánchez A, Vila A, Soriano I I, Evora C, Vila-Jato JL, Alonso MJ. The role of PEG on the stability in digestive fluids and in vivo fate of PEG-PLA  nanoparticles following oral administration. Colloids Surf B Biointerfaces. 2000;18(3-4):315-323. https://doi.org/10.1016/S0927-7765(99)00157-5

Soriano I, Evora C. Formulation of calcium phosphates/poly (d,l-lactide) blends containing gentamicin for bone implantation. J Control Release. 2000;68(1):121-34. https://doi.org/10.1016/S0168-3659(00)00251-0

Diaz RV, Llabrés M, Evora C. One-month sustained release microspheres of 125I-bovine calcitonin. In vitro-in vivo studies. J Control Release. 1999;59(1):55-62. PubMed PMID: 10210722. https://doi.org/10.1016/S0168-3659(98)00179-5

Evora C, Soriano I, Rogers RA, Shakesheff KN, Hanes J, Langer R. Relating the phagocytosis of microparticles by alveolar macrophages to surface chemistry: the effect of 1,2-dipalmitoylphosphatidylcholine. J Control Release. 1998;51(2-3):143-52. https://doi.org/10.1016/S0168-3659(97)00149-1

Shakesheff KM, Evora C, Soriano I I, Langer R. The Adsorption of Poly(vinyl alcohol) to Biodegradable Microparticles Studied by X-Ray Photoelectron Spectroscopy (XPS). J Colloid Interface Sci. 1997;185(2):538-47. https://doi.org/10.1006/jcis.1996.4637

Alarcó A, Bordallo AJ, Evora C, Llabrés M, Meneses MM, González Hermoso F. The bioavailability of drugs administered via a minimal jejunostomy catheter. A  clinico-experimental study. Nutr Hosp. 1995;10(3):181-4.

Cadórniga R, Molina IT, Pastoriza P, Negro S, Evora CM, Gutierrez JA. Pharmacokinetics of cefroxadine after infusion to healthy volunteers. Int J Clin Pharmacol Ther Toxicol. 1990;28(10):435-9.

Sánchez E, Evora CM, Torres A, Llabrés M. A new approach to pharmacokinetic parameters: estimation of cefuroxime during haemodialysis. Biopharm Drug Dispos.  1990;11(2):107-20.