Responsible: Dr. José M. Padrón
We perform the biological evaluation of synthetic and natural compounds against a panel of representative human solid tumour cell lines. From this test, we determine if compounds are active and/or interesting to go into depth. We try to shed light on the mechanism of action by studying the phenotypic effects of the products at a molecular level. Our ultimate goal is to identify the biological target(s) at which the active compound is directed.
Our evaluation system consists of three stages. It begins with a primary test to determine the antiproliferative capacity, and is followed by secondary biochemical and cellular tests, concluding with a confirmatory test. The assays are based on human cell cultures and result in sets of biologically relevant parameters (gene transcription, protein expression, etc.). To date, more than 5,500 compounds of both natural and synthetic origin have been evaluated.