Natural products are one of the most important sources of compounds with potential pharmaceutical applications. The prospecting of plant species capable of producing natural products with antiproliferative activity is one of the lines of research that has the greatest impact on the health and well-being of humanity. In this sense, secondary metabolites of plant origin have been a source of chemical entities with enormous potential for the treatment of human diseases. We provide our expertise in the isolation and characterization of secondary metabolites with potential pharmacological application.